| 摘要: |
| 通过在人工饲料中添加不同浓度Zn2+(75,150,300,600,1 200 mg/kg),研究了饲料中不同浓度Zn2+在连续2代不同发育阶段黑水虻体内的积累及幼虫不同组织的分布规律.结果表明,过量的Zn2+可在6龄幼虫、蛹和成虫体内积累,且Zn2+含量为6龄幼虫＞成虫＞蛹,然而,同一世代不同发育阶段虫体内的Zn2+含量不存在显著差异;但第2代受高剂量Zn2+胁迫虫体内Zn2+含量均显著高于第1代.Zn2+可在黑水虻5龄末幼虫表皮、脂肪体及中肠中累积,连续2代分布规律均为中肠＞脂肪体＞表皮,第2代各组织中Zn2+含量均高于第1代;在300 mg/kg Zn2+浓度下,第2代5龄末幼虫表皮和脂肪体中的Zn2+浓度与第1代存在显著差异,在高剂量(600和1 200 mg/kg) Zn2+处理浓度下,中肠中Zn2+含量显著高于第1代. |
| 关键词: Zn2+ 黑水虻 累积 分布 |
| DOI： |
| 分类号:Q969.97 |
| 基金项目:国家自然科学基金(31040075,31260528);广东省医学科研基金(A2012621) |
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| Accumulation and distribution of heavy metal Zn2+ in two successive generations black soldier fly Hermetia illucens L. (Dipetra: Stratiomyidae) |
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XIA Qiang1,LIAO Ye1,ZHU Wei1,YU Guo-hui2,CHEN Yuan-feng21,2
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1. Zunyi Medical College Zhuhai Campus ,Zhuhai 519041,China;2. Zhuhai Agriculture Research Center ,Zhuhai 519075, China
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| Abstract: |
| Artificial diets added with different concentrations of Zn2+(75, 150, 300, 600, 1 200 mg/kg) were fed on black soldier fly Hermetia illucens L. larvae for 2 generations, and contents of Zn2+ accumulation in different developmental stages of H. illucens and distribution in different tissues of the 5thinstar larvae were detected. Results showed that excessive Zn2+ accumulate in the 6th instar larvae , pupae and adults, and the order of Zn2+ contents in different stages is 6 instar larvae > adults > pupae. However, no significant difference is occurred in the different developmental stages’ H. illucens from the same generation. At the same time, Zn2+ contents from ones stressed with high-levels of Zn2+ in the 2ndgeneration are significantly higher than those in the 1st generation. Zn2+ accumulate in epidermis, fat body and midgut of the 5thinstar larvae. In the 2 successive generations, Zn2+ content in different tissues is in the order of midgut > fat body > epidermis, and the Zn2+ contents in 2nd generation are all significantly higher than those in the 1stgeneration. Furthermore, Zn2+contents in epidermis and fat body of the 2nd generation are significantly higher than those in the 1stgeneration under treatment with 300 mg/kg Zn2+. Significantly higher Zn2+ contents are found in midgut of 600 and 1 200 mg/kg Zn2+ stressed larvae from the 2ndgeneration when comparing to the 1st generation. |
| Key words: Zn2+ Hermetia illucens accumulation distribution |
