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| 熊果苷抑制斑马鱼胚胎黑色素合成的研究 |
| Research of arbutin inhibits melanin synthesis in zebrafish embryos |
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| DOI： |
| 中文关键词: 熊果苷 斑马鱼胚胎 黑色素 |
| 英文关键词: arbutin zebrafish embryos melanin |
| 基金项目:国家自然科学基金资助项目(20907042, 21277126)；浙江省自然科学基金资助项目(Y5090252) |
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| 中文摘要: |
| 为探讨美白化学品熊果苷对黑色素合成的抑制效果，以斑马鱼胚胎为模型，将其暴露于不同浓度的熊果苷溶液后，观察其黑色素生成情况，并进一步测定体内黑色素合成通路上部分关键基因的表达.定性观察表明，随着熊果苷浓度的增加，其对黑色素生成的抑制明显增强.基因表达分析表明，熊果苷通过对眼皮肤白化病II型（OCA2）, 酪氨酸酶（TYR）和银色毛发（SILV）等黑色素合成相关基因的抑制，进而抑制了黑色素的合成.上述结果解释了熊果苷在黑色素抑制过程中的作用机理，为斑马鱼胚胎模型在美白物质筛选中的应用提供了基础. |
| 英文摘要: |
| In order to investigate the inhibitory effect of one of the whitening chemicals arbutin on melanin synthesis, zebrafish embryos was used as a model, after exposing them to different concentrations of arbutin solution, the melanin synthesis were observed, furthermore expressions of several crucial genes on the melanin synthesis pathway in the embryos were measured. The results of qualitative observation showed that, with the increasing concentration of arbutin, its inhibition on melanin synthesis is significantly enhanced. The gene expression analysis showed that, arbutin inhibit the melanin synthesis by suppressing the expression of melanin synthesis-related genes, such as oculocutaneous albinism type 2 (OCA2), tyrosinase(TYR) and silver (SILV). These results explain the mechanism in melanin inhibiting process of arbutin, and provide a basis for the application of zebrafish embryo model in whitening chemicals screening. |
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