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| 白皮杉醇、白黎芦醇和赤松素与BSA相互作用的荧光光谱法研究 |
| Study on the interaction between flavonoid components of piceatannol, resveratrol, pinosylvin and bovine serum albumin by fluorescence spectroscopy |
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| DOI： |
| 中文关键词: 白皮杉醇 白藜芦醇 赤松素 牛血清白蛋白 荧光光谱法 |
| 英文关键词: piceatannol resveratrol pinosylvin bovine serum albumin fluorescence spectroscopy |
| 基金项目:湖南省科技厅自然科学研究基金(2011FJ6003) |
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| 中文摘要: |
| 应用荧光光谱法和紫外光谱法对二苯乙烯类化合物白皮杉醇(Piceatannol)、白藜芦醇(Resveratrol)和赤松素(Pinosylvin)与BSA之间的相互作用进行研究.计算了这3种化合物与BSA的结合常数和结合位点数及热力学函数△G,△S,△H.并分析了它们对BSA的荧光淬灭过程及其之间的相互作用类型,运用了Foerster’s偶极-偶极非辐射能量转移原理测定了与牛血清白蛋白的结合距离.结果表明,白皮杉醇、白藜芦醇和赤松素3种二苯乙烯化合物对BSA的荧光淬灭属于自发的以疏水作用力为主导的非辐射能量转移的静态淬灭过程.随分子中B环上的羟基数目的逐渐增加,白皮杉醇、白藜芦醇和赤松素与BSA的结合力逐渐增强. |
| 英文摘要: |
| The interactions between BSA and three stilbene compounds, piceatannol, resveratrol and pinosylvin were studied by means of fluorescence spectroscopy. The respective bingding constant Ka and number of binding sites of each compound were calculated as well as thermodynamic function △G,△S,△H .The bingding distance between each and BSA was obtained by Foerster′s dipile-dipile non-radiation energy transfer mechanism. The result showed that the binding of each compound with BSA proceeds spontaneously, and is mainly dominated by hydrophobic forces. Non-radiation energy transfer occurre between BSA with each compound. The structure of the three compounds influences significantly the binding process and the affinity increase with the numbers of -OH groups on the Bring. |
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