|
| 光谱法研究三磷酸腺苷二钠与牛血清白蛋白的相互作用及共存金属离子的影响 |
| Study on the Interaction and between Adenosine Disodium Triphosphate with Bovine Serum Albumin and the Effect of Coexistent Metal Ions on the Reaction by Spectroscopic Methods |
| 投稿时间：2014-10-24 修订日期：2014-10-24 |
| DOI： |
| 中文关键词: 三磷酸腺苷二钠 相互作用 金属离子 |
| 英文关键词: Adenosine Disodium Triphosphate interaction metal ions |
| 基金项目:云南省教育厅科研项目(2012Y414) ；国家自然科学基金资助项目(21261019)。 |
|
| 摘要点击次数: 2049 |
| 全文下载次数: 0 |
| 中文摘要: |
| 在模拟人体生理条件下,用紫外-可见吸收光谱法和荧光光谱法研究三磷酸腺苷二钠 (ADT)和牛血清白蛋白(BSA)的相互作用及共存金属离子Mn2+、Co2 、Cr3 、Mg2 、Fe3 对两者结合作用的影响。结果表明：ADT对BSA的荧光有猝灭作用,其猝灭过程属于动态猝灭。通过计算得出ADT与BSA的结合常数Kb及结合为点数n。根据热力学参数确定了ADT和BSA之间的作用力类型主要为静电引力。ADT在BSA中的结合位点主要位于亚螺旋域ⅡA。Hill系数nHill≈1,表明ADT对两者的结合几乎无协同作用。用同步荧光光谱法研究了ADT对BSA构象的影响。此外还详细探讨了共存金属离子对ADT与BSA结合作用的影响。 |
| 英文摘要: |
| Under the simulation of the human body physiological conditions,the interaction between Adenosine Disodium Triphosphate (ADT) and bovine serum albumin (BSA) was studied with UV-visible absorption spectra and fluorescence spectra. The effects of metal ions Mn2+、Co2 、Cr3 、Mg2 、Fe3 on the interaction of ADT with BSA were also discussed. The results showed that the fluorescence of BSA was quenched by ADT, which was a dynamic quenching process. The binding constants Kb and the corresponding number of binding sites n were obtained by calculation. The thermodynamic parameters obtained from measured data showed that the interaction of ADT and BSA was mainly driven by the electrostatic force. The primary binding site for ADT was located at sub-domain ⅡA of BSA. The values of Hill"s coefficients were nearly 1, which indicated that there was nearly no cooperative effect. The effect of ADT on the conformation of BSA was analyzed by synchronous fluorescence spectrometry. In addition, the effect of metal ions on the binding constant of ADT with BSA was also studied. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
