湖南科技大学学报（自然科学版）

文章摘要

食品废水A/O工艺的低温启动阶段膨胀污泥的微生物群落解析

Microbial community analysis of bulking sludge during food wastewater A/O process in low temperature

DOI：

英文关键词: food wastewater A/O process low-temperature startup sludge bulking community analysis

基金项目:国家自然科学青年基金资助项目（50908063）

作者	单位
魏利1,牛春革2,聂春梅2,陈爱华2,王传萍2,李春颖3,魏超1	1.哈尔滨工业大学城市水资源与水环境国家重点实验室，黑龙江哈尔滨 150090； 2.中国石油克拉玛依石化分公司炼油化工研究院，新疆克拉玛依834000； 3.哈尔滨商业大学能源建筑与工程学院，黑龙江哈尔滨 150028

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中文摘要:

污泥膨胀是污水处理工艺启动和运行中棘手的问题，食品废水采用A/O工艺进行低温下启动运行时，出现了污泥膨胀的现象，研究对A/O工艺段水质进行了分析，构建了16S rDNA克隆文库.通过对膨胀污泥微生物群落解析，探讨了污泥膨胀和工艺调控的关系.研究表明，厌氧膨胀污泥中Proteobacteria占总的细菌数的63.51%，Chloroflexi为16.22%，其中Thauera sp. 占到总的细菌数的20.27%；好氧膨胀污泥中 Proteobacteria 占总的细菌数的36.08%，Chloroflexi为24.74%，Bacteroidetes为7.22%，出现了与污泥膨胀有关的细菌Uncultured Nocardia，Uncultured Leptolyngbya sp.，Thauera sp..导致污泥膨胀的主要原因是工艺的进水温度低，厌氧段厌氧程度不够，可生化性差，部分重金属超标，好氧段的曝气量不足.建议通过调控工艺的进水温度，提高厌氧段的厌氧程度，提高好氧段的曝气量，可以有效地控制污泥膨胀.

英文摘要:

It is difficult to resolve the problem of sludge bulking. The A/O process was applied for the food wastewater treatment. It was found that sludge expanded in the low temperature. The water quality was analyzed, and 16S rDNA clone library was built. Through the analysis the microbial community, the relationship of sludge bulking and process control was studied. It showed that the percent of Proteobacteria is 63.51%, Chloroflexi is 16.22% and Thauera sp. is 20.27% in anaerobic sludge bulking. The percent of Proteobacteria was 36.08%, Chloroflexi was 24.74% and Bacteroidetes is 7.22% in aerobic sludge bulking. In the same time, Uncultured Nocardia, Uncultured Leptolyngbya sp., Thauera sp. are found which are related with sludge bulking. The low temperature is the main external ecological factors. There are several reasons to cause the sludge bulking, such as the lack of anaerobic degree in anaerobic period , poor biochemical, high concentration of heavy metal and oxygen deficiency in aerobic period. It is showed that sludge bulking will be control effectively when the process of inlet temperature was regulateed, improve the anaerobic degree and aeration fluxes.

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