| 摘要: |
| 利用环烷酸、二乙烯三胺或三乙烯四胺、硫酸二乙酯为原料合成了2种咪唑啉季铵盐M1和M2，M1，M2分别在pH=2.36和pH=3.78的Britton- Robinson缓冲溶液中与fsDNA相互作用后，体系的共振光散射(RLS)强度明显增强.研究了它们与fsDNA作用的影响因素，在优化条件下，RLS强度增加值与fsDNA浓度之间呈良好的线性关系，拟定了新的测定fsDNA的RLS法.该法具有灵敏度高，操作简单，准确度好，线性范围宽等特点，用于合成样的测定，结果满意. |
| 关键词: 咪唑啉 咪唑啉季铵盐 fsDNA 共振光散射(RLS) |
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| 基金项目:湖南省教育厅资助项目（12k102）；湖南省大学生研究性学习和创新性实验计划项目（KDCX0804） |
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| The interaction between imidazolinyl quaternary ammonium salt and fsDNA and its analytical applications using resonance light scattering spectroscopy |
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| Abstract: |
| Two imidazolinyl quaternary ammonium salt M1 and M2 were synthesised using naphthenic acid, diethylene triamine (or triethylene tetramine), and diethyl sulfate. The interaction between fsDNA respectively with M1 and M2, enhanced the intensities of resonance light scattering (RLS) significantly in Britton Robinson buffer solution of the pH 2.36 for M1 and 3.78 for M2. Also, it was studied the effect factors on the reaction between imidazolinyl quaternary ammonium salt and fsDNA. Under the optimum conditions, the enhanced intensities of RLS had good linear relationship with the concentration of fsDNA. Thus, a new RLS method for determination of fsDNA was established. The method has the properties of high sensitivity, simple operation, good accuracy and the wide range of linear. It can be used to the determination of fsDNA containin the synthesized samples with the result of satisfying. |
| Key words: imidazoline, imidazolinyl quaternary ammonium salt, fsDNA, resonance light scattering |
