The analysis of temperature gradient effect special-shaped box girder is rear, both in China and abroad. Take a cable-stayed arch special-shaped box girder bridge for example, four conditions were taken according to four kinds of typical temperature gradient models, finite element software MIDAS/Civil was used to calculate the temperature stress causing by temperature gradient of the control sections. The sensitivity of temperature stress was analyzed, that about the temperature gradient models and exploring the change rule of the temperature stress value in the special-shaped beam section height and the longitudinal of the bridge. The results show that the maximum temperature stress in the longitudinal of the special-shaped box girder appears in the variable cross-section place. Temperature stress value under the temperature gradient model of New Zealand concrete design standard is the largest. The temperature gradient model in 04 standard has higher security reserves than that’s in 85 standard. Temperature stress in the special-shaped box girder cross section is very sensitive about the temperature gradient model, so choosing the local temperature gradient model at that time is very important.