Artificial diets added with different concentrations of Zn2+(75, 150, 300, 600, 1 200 mg/kg) were fed on black soldier fly Hermetia illucens L. larvae for 2 generations, and contents of Zn2+ accumulation in different developmental stages of H. illucens and distribution in different tissues of the 5thinstar larvae were detected. Results showed that excessive Zn2+ accumulate in the 6th instar larvae , pupae and adults, and the order of Zn2+ contents in different stages is 6 instar larvae > adults > pupae. However, no significant difference is occurred in the different developmental stages’ H. illucens from the same generation. At the same time, Zn2+ contents from ones stressed with high-levels of Zn2+ in the 2ndgeneration are significantly higher than those in the 1st generation. Zn2+ accumulate in epidermis, fat body and midgut of the 5thinstar larvae. In the 2 successive generations, Zn2+ content in different tissues is in the order of midgut > fat body > epidermis, and the Zn2+ contents in 2nd generation are all significantly higher than those in the 1stgeneration. Furthermore, Zn2+contents in epidermis and fat body of the 2nd generation are significantly higher than those in the 1stgeneration under treatment with 300 mg/kg Zn2+. Significantly higher Zn2+ contents are found in midgut of 600 and 1 200 mg/kg Zn2+ stressed larvae from the 2ndgeneration when comparing to the 1st generation.