Cervical cancer Hela cells were treated with mango polyphenols of the different concentrations, and then the cell survival rate was detected by MTT method, cell apoptosis and cell cycle distribution were tested through Hoechst 33258 fluorescent staining and flow cytometry, p53,p21,c-myc,cyclin D1 protein level change were determined by Western blot. The results showed that mango polyphenols (0.025 mg/ml, 0.05 mg/ml, 0.1 mg/ml, 0.2mg/ml, and 0.4 mg/ml) obviously decrease the survival rate of Hela cells in a time and dose dependent manner; there are visible thick fluorescent particles in most of fluorescent staining cell and cell morphology change such as nuclear shrinkage; The number of G1 phase Hela cells compared with control group are significantly increased and the number of G2 phase cells gradually reduced; Cell apoptosis rate is significantly higher than those in the control group; Mango polyphenols increased protein levels of p53, p21 and decreased protein levels of c-myc, cyclin D1 in Hela cells. The above results showed that mango polyphenols obviously inhibit the proliferation of Hela cells and induce its apoptosis.