The interactions between BSA and three stilbene compounds, piceatannol, resveratrol and pinosylvin were studied by means of fluorescence spectroscopy. The respective bingding constant Ka and number of binding sites of each compound were calculated as well as thermodynamic function △G,△S,△H .The bingding distance between each and BSA was obtained by Foerster′s dipile-dipile non-radiation energy transfer mechanism. The result showed that the binding of each compound with BSA proceeds spontaneously, and is mainly dominated by hydrophobic forces. Non-radiation energy transfer occurre between BSA with each compound. The structure of the three compounds influences significantly the binding process and the affinity increase with the numbers of -OH groups on the Bring.