The total amount of oxygen content and inclusions in 50Cr5MoV forged steel roll by the route of EBT→LF→VD were analyzed . The results show the average ω(T［O］) is 47×10-6 after LF refining and 14×10-6after VD refining ,and 15.5×10-6 in tundish and 18×10-6 in billet on average. During the initial stage of LF refining, the inclusions in steel mainly are irregular forms of Al2O3, 96.75% of the inclusions have diameters less than 10 μm. After the LF refining, most of the inclusions are 0～10 μm diameters of compound oxide inclusions, predominantly containing CaOAl2O3SiO2.During the transfer of steel to tundish,the protective casting is not ideal, and the severe reoxidation contributes to the growing amount of inclusions, mainly containing spherical mCaO·nAl2O3compound oxide inclusions.99.81 percent of the inclusions in the steel slab are less than 10 μm.Most of them are spherical calcium-aluminate compound inclusion, with a few spherical calcium aluminosilicate compound inclusion. Therefore, inclusion control in 50Cr5MoV steel is a systematic engineering and could not be achieved by partial technical improvement.